To localize glycoconjugates in the testis of SAM(senescence accelerated mice) and SRM (senescence resistant mice), seven different biotinylated lectins(SBA, DBA, PNA, BSL-1, sWAG, USE-1 and Con A1 were applied with ABC method.
The cell surface of spermatogonia and primary spermatocytes showed an affinity for PNA and sWGA, and an additional DBA and SBA to those found in the cell surface were observed in the Golgi region of primary spermatocytes. The developing
spermatids
in
transition from Golgi to acrosome phase, reactivity to SBA and BSL-1 were detected in acrosome, PNA and Con A in cytoplasm, and PNA and sWGA in cell surface. In the spermatids of maturation phase, however, affinity for DBA, PNA, sWGA and Con A
was
observed only in the tail. The staining was observed in the cytoplasmic droplet of developing spermatids with all lectin examined except BSL-1 and UEA-1. PNA, BSL-1, sWGA and Con A staining showed in the cytoplasmic process of Sertoli cells.
Both SAM and SRM showed a similar affinity for all lectins examined.
|